Oligonucleotide transcription factor decoys as tools to control bacterial transcription

Transcription factor proteomics-Tools, applications, and challenges.

Transcription factors (TFs) are a family of DNA-binding proteins whose gene regulatory capabilities are of vital importance in defining the molecular state of a cell. Despite their biological significance, our understanding of TF behavior and function is still limited. This is because we have so far mostly relied on gene expression data to approximate TF protein levels given that the latter inf...

Blimp-1 Expression as an Exhaustion Transcription Factor in Chronic Lymphocytic Leukemia

Background: PPreviously, it was shown that exhausted CD4+ and CD8+ T cells in chronic lymphocytic leukemia (CLL) co-express the two immune-inhibitory receptors, Tim-3 and PD-1. Present study investigated the expression of Blimp-1, a transcription factor involved in T cell exhaustion, in patients with CLL. Materials and Methods: Peripheral blood mononuclear cells were collected from 25 untreate...

Yeast genetic selections to optimize RNA decoys for transcription factor NF- B

In vitro-selected RNA aptamers are potential inhibitors of diseaserelated proteins. Our laboratory previously isolated an RNA aptamer that binds with high affinity to human transcription factor NFB. This RNA aptamer competitively inhibits DNA binding by NFB in vitro and is recognized by its target protein in vivo in a yeast three-hybrid system. In the present study, yeast genetic selections wer...

metabolic syndrome and mitochondrial transcription factor a

Protein‐Functionalized DNA Nanostructures as Tools to Control Transcription in Zebrafish Embryos

The unique structure-directing properties of DNA origami nanostructures (DONs) show great potential to specifically manipulate intracellular processes. We report an innovative concept to selectively activate the transcription of a single gene in the developing zebrafish embryo. We reason that engineering a designer transcription factor in which a rigid DON imposes a fixed distance between the D...